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방사선조사가 저칼슘식이 백서의 하악골에 미치는 영향에 관한 실험적 연구
THE EFFECTS OF IRRADIATION ON THE MANDIBUIAR BONE OF RATS ON THE LOW CALCIUM DIET

치과방사선 1992년 22권 2호 p.241 ~ 257

김종효, 김태권,

소속 상세정보

김종효 ( ) - 경희대학교 식품공학과
김태권 ( ) - 경희대학교 치과대학 치과방사선학교실

KMID : 0378219920220020241

Abstract

저자는 저칼슘식이 골송조증을 유발시킨 백서의 하악골에 방사선을 조사한 후 골조직에서
일어나는 이의 구조와 형태의 변화를 구명하기 위하여 본 연구를 시행하였다.
실험동물로서는 7주령의 체중 150gm 내외의 Sprague-Dawley계 웅성백서 64마리를 채택
하였으며, 정상식 이군을 대조군으로 저칼슘식 이군을 실험군으로 정하였다. 이들 대조군과
실험군은 각각 방사선조사군과 방사선비조사군으로 다시 나누었으며, 이들 각군에 16마리씩
을 배정하였다. 대조군 및 실험군의 방사선조사군에는 10주령 에 흡수선량이 20Gy가 되도
록 Co-60 감마선을 단회 조사하였으며, 각군의 실험동물을 방사선조사 후 3일, 7일, 14일,
21일에 4마리씩 희생시킨 다음 양측 하악골을 적출하여 이들을 microradiography법과 병리
조직 및 면역세포확적 방법으로 관찰하였던 바, 다음과 같은 결과를 얻었다.
1. 저칼슘식이 방사선비조사군의 microradiogram상에서는 실험 전기간에 걸쳐 치밀골이
비박되었고, 치근간치조골 및 골체부의 골소주가 감소되었으며, 광학현미 경상에서도 실험
14일에는 골수 중앙부에서 골용해소견이 현저하였다.
2. 저칼슘식이 방사선조사군에서는 방사선비조사군에 비하여 microradiogram상에서 실험
7일부터 치밀골의 비박과 골소주의 감소가 뚜렷하였으며, 광학현미경상에서도 실험 7일에
골소주 변연부에서 조골세포가 현저히 감소되었고, 파골세포가 다수 관찰되었다.
3. 저칼슘식이 방사선조사군에서는 정상식이 방사선조사군에 비하여 microradiogram상에
서 실험 전기간에 걸쳐 치밀골의 비박과 골소주의 감소가 현저하였으며, 정상식이 방사선조
사군에서는 광학현미경상에서 골용해상을 관찰할 수 없었다.
4. 면역세포화학적 관찰에서 Tumor Necrosis Factor를 함유하고 있는 골수세포는 정상식
이 방사선조사군에서는 다수 관찰되었으나, 저 칼슘식이군에서는 미약하거라 관찰되지 않았다.
#초록#
The Purpose of this study was to investigate the changes of morphology and
structure of bone tissue in the irradiated mandibular bone in rats which were fed a low
calcium diet.
In order to carry out this experimant, 64 seven-week old Sprague-Dawley strain rats
weighing about 150gms were selected and equally divided into one experimental group
of 32 rats and one control group with the remainder.
The experimental group and the control group were then subdivided into two groups
when the rats reached ten-week old, 16 were assigned rats for each subdivided group,
exposed to irradiation. The two irradiation groups received a single dose of 20 Gy in the
jaws area only and irradiated with a cobalt-60 teletherapy unit The rats in the control
and experimental groups were serially terminated by fours on the 3rd, the 7th, the 14th,
and the 21st day after irradiation Following termination, both sides of the dead rats
mandibular bodies were removed and fried with 10% neutral formalin. One side of the
mandibular body was radiographer with a sort X-ray apparatus. Thereafter, the obtained
microradiographs were observed by a light microscope. The remaining side of the
mandibular bone was further decalcified and embedded in paraffin as using the general
method. The specimen sectioned and stained with hematoxylin and eosin, and Rabit
Anti-Human Tumor Necrosis Factor-α observed by a light microscope.
The obtained results were as follows:
1. Microradiogram revealed that thinning of the cortex and a decrease in the trabecula
of the interradicular bone and mandibular body were observed and noted from the start
to finish throughout the experiment in the non-irradiated rats on the low calcium diet
rather than in the non-irradiated rats on the normal diet
In microscopic observational there were marked osteolytic changes in the center of the
bone marrow.
2. Microradiosram revealed that thinning of the cortex and a decrease in the trabecula
of interradicular bone and mandibular body were more marked after 7 days in the
irradiated rats on the low calcium diet rather than in the non-irradiated rats on the low
clacium diet.
In microscopic observations, osteoblasts were decreased markedly after 7 days, and a
few osteoclasts were observed.
3. Microradiogram revealed that thinning of the cortex and a decrease in trabecula of
interradicular bone and the mandibular body were more marked from the start to finish
troughout the experiment in the irradiated rats on the low calcium diet rather than in
the irradiated rats on the normal diet.
In microscopic observations, there were no osteolytic changes noted in the irradiated
rats on the normal diet
4. In immunocytochemical finding, a few bone marrow cells including Tumor Necrosis
Factor were observed in the irradiated rats on the normal diet, but was not observed in
the rats on the low calcium diet.